Blood Bank Case Study

------------------------------------------------- Analytical Toxicology Background In analytical toxicology especially to analyze drugs, several(prenominal) rules great deal be apply. The manners can be categorized dish on their principle of assay which atomic number 18 chemical, spectrometric, chromatographic, electrophoretic and immunochemical assays. for each one principle of depth psychology populates of few techniques that can be used. However, immunoassay is normally used in drug screening neglectful to sensitivity, specificity and low bell than separate methods. The half-life of drug excessively used in toxicology analysis to helps in therapeutic prise and accessing drug abuse. Introduction Immunoassays are bioanalytical method in which the quantitation of analyte depends on the reaction of antigen (analyte) and antibody. (Ibrahim A. Darwish , 2006). Immunoassay principle is found on competitive click charge which specific antibody is used to detect the presence of analyte or its metabolite in the assay tested. This method consists of some techniques which include: I. Radioimmunoassay (RIA) II. Enzyme immunoassay (EIA) III. Enzyme figure immunoassay technique (EMIT) IV. Enyzme linked immunoasorbent assay (ELISA) V. Fluorescence polarization immunoassay (FPIA) VI. Cloned enzyme donor immunoassay (CEDIA) I.

Radioimmunoassay (RIA) This technique is no longer commonly used collect to waste judicature issues where high gear cost of handling and disposal cost. The analyte in the sample is detect victimization radioisotope. In RIA, sample is mixed with specific antibody to the knave ask analyte and tagged analyte with radioactive iodine. Analyte in the sample repugn with labeled analyte for antibody. da da Gamma counter is used to touchstone the labeled analyte bind with antibody per slim (CPM). II. Enzyme immunoassay (EIA) EIA is similar to RIA tho instead of measuring ray syndrome this technique measure enzyme activity. It consist of signal detection beat which the result is measured optically by fluorescence...If you want to nourish a full essay, put in it on our website: Orderessay

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